8WED

Crystal structure of Arabidopsis thaliana MIK2 ectodomain in complex with BAK1 ectodomain and Fusarium oxysporum SCOOPL

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2023-04-10
Detector	PSI PILATUS 6M
Wavelength(s)	0.97853
Spacegroup name	P 1 21 1
Unit cell lengths	72.674, 178.123, 142.263
Unit cell angles	90.00, 90.11, 90.00

Refinement procedure

Resolution	36.340 - 3.300
R-factor	0.2415
Rwork	0.240
R-free	0.26120
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.625
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.420
High resolution limit [Å]	3.300	3.300
Number of reflections	54443	1888
<I/σ(I)>	16.1
Completeness [%]	99.9
Redundancy	5.8
CC(1/2)	0.988	0.540

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	5.9	289	0.1 M MES pH 5.9, 22% PEG200, 7% PEG 3000