8WDI

Crystal structure of lysozyme by fixed-target pink-beam serial synchrotron crystallography

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 11C
Synchrotron site	PAL/PLS
Beamline	11C
Temperature [K]	295
Detector technology	PIXEL
Collection date	2023-05-23
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.8366
Spacegroup name	P 43 21 2
Unit cell lengths	78.830, 78.830, 38.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.990 - 2.200
R-factor	0.2561
Rwork	0.251
R-free	0.29930
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.969
Data reduction software	CrystFEL
Data scaling software	CrystFEL
Phasing software	MOLREP
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	79.360	2.270
High resolution limit [Å]	2.200	2.200
Number of reflections	6525	628
<I/σ(I)>	5.43
Completeness [%]	100.0
Redundancy	492.9
CC(1/2)	0.874	0.524

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	SMALL TUBES		295	Na-acetate, NaCl, PEG8000