8WDG
Subatomic crystal structure of glucose isomerase from Streptomyces rubiginosus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 11C |
| Synchrotron site | PAL/PLS |
| Beamline | 11C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-12-09 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.8265 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 92.675, 98.626, 102.137 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.770 - 0.990 |
| R-factor | 0.15874 |
| Rwork | 0.158 |
| R-free | 0.17071 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.043 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.770 | 1.010 |
| High resolution limit [Å] | 0.990 | 0.990 |
| Number of reflections | 255970 | 12132 |
| <I/σ(I)> | 12.2 | |
| Completeness [%] | 99.5 | 94.6 |
| Redundancy | 6.1 | |
| CC(1/2) | 0.999 | 0.561 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | Tris-HCl, pH 8.0, PEG400, MgCl2 |
