8WD3
The Crystal Structure of JMJD2A(M1-L359) from Biortus.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08B1-1 |
| Synchrotron site | CLSI |
| Beamline | 08B1-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-08-27 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 1.18057 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 51.641, 97.111, 141.083 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.603 - 3.300 |
| Rwork | 0.244 |
| R-free | 0.29280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.025 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.603 | 3.560 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.162 | 0.675 |
| Number of reflections | 11222 | 2264 |
| <I/σ(I)> | 6.9 | |
| Completeness [%] | 99.8 | |
| Redundancy | 5.6 | |
| CC(1/2) | 0.996 | 0.867 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.2M KSCN, 0.1M Bis-Tris propane pH7.5, 20% PEG 3350 |
