8WCG

Crystal structure of SARS-CoV-1 RBD in complex with nanobody aSR29 and aSR347

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	103.15
Detector technology	PIXEL
Collection date	2022-08-26
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979021
Spacegroup name	P 21 21 21
Unit cell lengths	76.852, 134.679, 136.688
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.967 - 2.599
R-factor	0.2346
Rwork	0.232
R-free	0.27930
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond angle	0.634
Data reduction software	autoPROC
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX ((dev_2481: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	95.930	2.740
High resolution limit [Å]	2.599	2.600
Rmerge	0.121	1.191
Rmeas	0.129
Number of reflections	83452	6390
<I/σ(I)>	15.1
Completeness [%]	99.1	99.7
Redundancy	7.7	7.97
CC(1/2)	0.998	0.784

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	20% w/v PEG4000, 0.1 M Tris 8.0