8WB8
Crystal Structure of the shaft pilin LrpA from Ligilactobacillus ruminis - orthorhombic form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-10-30 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.972423 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 62.416, 165.240, 47.928 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 58.460 - 2.300 |
R-factor | 0.21789 |
Rwork | 0.216 |
R-free | 0.26161 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 1.253 |
Data reduction software | autoPROC |
Data scaling software | autoPROC |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 58.460 | 2.468 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.139 | 0.899 |
Rmeas | 0.148 | 0.964 |
Rpim | 0.052 | 0.345 |
Number of reflections | 15406 | 770 |
<I/σ(I)> | 8.1 | |
Completeness [%] | 94.4 | 91.9 |
Redundancy | 8.1 | 7.5 |
CC(1/2) | 0.997 | 0.783 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | 100 mM Sodium Acetate, pH 4.5, 20% w/v Polyethylene Glycol 3000 |