8WAI

Structure of RvY_06210 at 1.45 angstrom resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-06-16
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9
Spacegroup name	P 21 21 21
Unit cell lengths	60.960, 82.161, 145.065
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.580 - 1.450
R-factor	0.169
Rwork	0.168
R-free	0.19220
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.842
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.960	1.470
High resolution limit [Å]	1.450	1.450
Rmerge	0.059	1.320
Rmeas	0.064	1.431
Number of reflections	129382	6276
<I/σ(I)>	16.1
Completeness [%]	99.8
Redundancy	6.7
CC(1/2)	0.999	0.586

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.4M Sodium thiocyanate, 0.1M Sodium acetate pH4.0, 16% w/v PEG 4000