8W9J

Crystal structure of human CLEC12A ectodomain complexed with 50C1 Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	95
Detector technology	PIXEL
Collection date	2023-05-23
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.98
Spacegroup name	C 1 2 1
Unit cell lengths	165.356, 45.142, 202.749
Unit cell angles	90.00, 93.13, 90.00

Refinement procedure

Resolution	42.130 - 3.500
R-factor	0.2557
Rwork	0.254
R-free	0.29130
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.550
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.540	3.830
High resolution limit [Å]	3.500	3.500
Rpim	0.115	0.328
Number of reflections	19367	4549
<I/σ(I)>	4.8
Completeness [%]	99.3
Redundancy	3.4
CC(1/2)	0.981	0.773

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.1 M HEPES-Na (pH 7.5), 0.2 M sodium chloride, 12% (w/v) PEG8000