8W94

Azumapecten Farreri homopolymeric ferritin (ApF) mutant-H2KE exposed to H2O2 for 2 s

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-04-01
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0000
Spacegroup name	I 4 3 2
Unit cell lengths	152.652, 152.652, 152.652
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.940 - 2.300
R-factor	0.1643
Rwork	0.160
R-free	0.19980
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.796
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.940	2.380
High resolution limit [Å]	2.300	2.300
Number of reflections	13688	1313
<I/σ(I)>	18.12
Completeness [%]	99.0
Redundancy	36.6
CC(1/2)	0.987	0.760

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100 mM MOPS, pH7, 500 mM NaCl