8W7R

H. walsbyi bacteriorhodopsin mutant - W94F

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE TPS 07A
Synchrotron site	NSRRC
Beamline	TPS 07A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-05-31
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.9762
Spacegroup name	P 1 21 1
Unit cell lengths	49.499, 126.747, 55.037
Unit cell angles	90.00, 111.44, 90.00

Refinement procedure

Resolution	26.960 - 2.503
Rwork	0.213
R-free	0.25670
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.400
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.581	2.640
High resolution limit [Å]	2.500	2.500
Rmeas	0.097	0.724
Number of reflections	21677	3427
<I/σ(I)>	14.22	2.14
Completeness [%]	99.6	99
Redundancy	3.1
CC(1/2)		0.730

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	5.5	293	50mM Sodium acetate, 50mM NaCl, 15% PEG 400