8W5C
Crystal structure of FGFR4 kinase domain in complex with 8K
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CAMD BEAMLINE GCPCC |
Synchrotron site | CAMD |
Beamline | GCPCC |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-10-09 |
Detector | DECTRIS PILATUS 300K |
Wavelength(s) | 0.987 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 61.831, 61.799, 181.621 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.640 - 2.300 |
R-factor | 0.2308 |
Rwork | 0.229 |
R-free | 0.26400 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.661 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 55.000 | 2.383 |
High resolution limit [Å] | 2.300 | 2.301 |
Number of reflections | 31513 | 30990 |
<I/σ(I)> | 7.1 | |
Completeness [%] | 95.3 | |
Redundancy | 2.383 | |
CC(1/2) | 0.990 | 0.992 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 296 | Na citrate ph 5.5, (NH4)2SO4 1.4M |