8W5C

Crystal structure of FGFR4 kinase domain in complex with 8K

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CAMD BEAMLINE GCPCC
Synchrotron site	CAMD
Beamline	GCPCC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-10-09
Detector	DECTRIS PILATUS 300K
Wavelength(s)	0.987
Spacegroup name	P 21 21 21
Unit cell lengths	61.831, 61.799, 181.621
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.640 - 2.300
R-factor	0.2308
Rwork	0.229
R-free	0.26400
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.661
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.000	2.383
High resolution limit [Å]	2.300	2.301
Number of reflections	31513	30990
<I/σ(I)>	7.1
Completeness [%]	95.3
Redundancy	2.383
CC(1/2)	0.990	0.992

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	296	Na citrate ph 5.5, (NH4)2SO4 1.4M