8W4L
Crystal structure of closed conformation of human immunoglobulin Fc in presence of EndoSz
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE TPS 05A |
Synchrotron site | NSRRC |
Beamline | TPS 05A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-10-28 |
Detector | RAYONIX MX300-HS |
Wavelength(s) | 1 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 151.964, 151.964, 113.300 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 28.720 - 3.100 |
R-factor | 0.2092 |
Rwork | 0.207 |
R-free | 0.25690 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.720 | 3.211 |
High resolution limit [Å] | 3.100 | 3.101 |
Number of reflections | 14294 | 1265 |
<I/σ(I)> | 10.4 | |
Completeness [%] | 98.6 | |
Redundancy | 11.2 | |
CC(1/2) | 0.963 | 0.742 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 100 mM MES PH 6.0 200 mM Zinc acetate 10 % PEG 8000 |