8W31

Crystal structure of parkin (R0RB):2pUb with activator compound

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-04-19
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.953
Spacegroup name	P 61 2 2
Unit cell lengths	83.540, 83.540, 258.770
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.220 - 2.500
R-factor	0.2423
Rwork	0.240
R-free	0.29370
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.281
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX (1.20.1-4487)
Refinement software	PHENIX (1.20.1-4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.220	2.590
High resolution limit [Å]	2.500	2.500
Rmerge	0.110	2.938
Rmeas	0.112	2.980
Rpim	0.018	0.464
Number of reflections	19414	1881
<I/σ(I)>	24.08	1.72
Completeness [%]	100.0	100
Redundancy	38.3	40.7
CC(1/2)	1.000	0.718

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	277	0.2 M Sodium Chloride, 0.1M HEPES pH 7.5, 25% (w/v) PEG 3350