8W31
Crystal structure of parkin (R0RB):2pUb with activator compound
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-04-19 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.953 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 83.540, 83.540, 258.770 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.220 - 2.500 |
R-factor | 0.2423 |
Rwork | 0.240 |
R-free | 0.29370 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.281 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX (1.20.1-4487) |
Refinement software | PHENIX (1.20.1-4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.220 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.110 | 2.938 |
Rmeas | 0.112 | 2.980 |
Rpim | 0.018 | 0.464 |
Number of reflections | 19414 | 1881 |
<I/σ(I)> | 24.08 | 1.72 |
Completeness [%] | 100.0 | 100 |
Redundancy | 38.3 | 40.7 |
CC(1/2) | 1.000 | 0.718 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 277 | 0.2 M Sodium Chloride, 0.1M HEPES pH 7.5, 25% (w/v) PEG 3350 |