8W2W
Structure of transthyretin synthetic mutation A120L
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-11-24 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0332 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 42.141, 64.069, 84.633 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.030 - 2.070 |
| R-factor | 0.2124 |
| Rwork | 0.210 |
| R-free | 0.23550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.548 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.21rc1_5127) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.030 | 2.140 |
| High resolution limit [Å] | 2.070 | 2.070 |
| Rmerge | 0.074 | 0.628 |
| Rmeas | 0.075 | 0.703 |
| Rpim | 0.022 | 0.310 |
| Number of reflections | 7206 | 361 |
| <I/σ(I)> | 15.1 | 3.2 |
| Completeness [%] | 99.7 | 98.6 |
| Redundancy | 5.6 | 8.5 |
| CC(1/2) | 0.998 | 0.889 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.1 M sodium cacodylate (pH 6.5), 1M sodium chloride, 10% (v/v) glycerol, and 30% (v/v) PEG 600 |
