8W0X

Crystal structure of broadly neutralizing antibody hcab40 in complex with Hepatitis C virus envelope glycoprotein E2 ectodomain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	298
Detector technology	PIXEL
Collection date	2021-12-09
Detector	DECTRIS PILATUS3 X 6M
Wavelength(s)	0.98
Spacegroup name	P 32 2 1
Unit cell lengths	79.911, 79.911, 294.567
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.040 - 3.120
R-factor	0.2287
Rwork	0.227
R-free	0.27050
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.293
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.040	3.030
High resolution limit [Å]	2.870	2.870
Rmerge	0.204	5.876
Rmeas	0.209	6.025
Rpim	0.047	1.321
Total number of observations	511303	76157
Number of reflections	25984	3711
<I/σ(I)>	8.9	0.7
Completeness [%]	99.9
Redundancy	19.7	20.5
CC(1/2)	0.999	0.451

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	298	0.1 M Sodium malonate and 12% PEG 3,350