8W0W

Crystal structure of broadly neutralizing antibody hcab64 in complex with Hepatitis C virus envelope glycoprotein E2 ectodomain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	298
Detector technology	PIXEL
Collection date	2021-07-21
Detector	DECTRIS PILATUS3 X 6M
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	68.680, 88.231, 174.709
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	53.990 - 2.130
R-factor	0.1963
Rwork	0.195
R-free	0.22410
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.082
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.21_5207)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	87.350	2.190
High resolution limit [Å]	2.130	2.130
Rmerge	0.163	3.063
Rmeas	0.179	3.342
Rpim	0.071	1.320
Total number of observations	365813	28522
Number of reflections	59648	4582
<I/σ(I)>	7.2	1.1
Completeness [%]	99.1
Redundancy	6.1	6.2
CC(1/2)	0.995	0.366

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	298	8% Tacsimate and 20% PEG 3,350