8VZY

Q108K:K40L:T51V:T53S:R58Y mutant of hCRBPII bound to synthetic fluorophore TD-1V

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-04-11
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.97872
Spacegroup name	P 1
Unit cell lengths	32.289, 35.252, 64.872
Unit cell angles	92.51, 90.49, 111.72

Refinement procedure

Resolution	29.200 - 1.340
Rwork	0.238
R-free	0.28200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4qyn
RMSD bond length	0.013
RMSD bond angle	1.632
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.16)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.200	1.370
High resolution limit [Å]	1.340	1.340
Number of reflections	47165	12003
<I/σ(I)>	43.37
Completeness [%]	79.6
Redundancy	2
CC(1/2)	0.837	0.862

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	PEG4000, ammonium acetate, sodium acetate