8VZX

Q108K:K40L:T53A:R58F mutant of hCRBPII bound to synthetic fluorophore TD-1V

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-09-17
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.97872
Spacegroup name	P 1
Unit cell lengths	36.451, 55.167, 69.876
Unit cell angles	109.94, 98.49, 101.41

Refinement procedure

Resolution	27.400 - 1.470
Rwork	0.233
R-free	0.26300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4qyn
RMSD bond length	0.007
RMSD bond angle	1.283
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.16)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.400	1.510
High resolution limit [Å]	1.470	1.470
Number of reflections	82805	16538
<I/σ(I)>	32.9
Completeness [%]	95.7
Redundancy	2.9
CC(1/2)	0.896	0.912

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	ammonium acetate, sodium acetate, PEG4000