8VYL
The structure of Human Hemoglobin in Complex with Nanobody BtNbE11
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-07-19 |
Detector | DECTRIS EIGER X 4M |
Wavelength(s) | 0.9537 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 188.761, 74.837, 57.875 |
Unit cell angles | 90.00, 91.44, 90.00 |
Refinement procedure
Resolution | 49.890 - 2.020 |
R-factor | 0.2108 |
Rwork | 0.208 |
R-free | 0.26590 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 0.723 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX ((1.20.1_4487: ???)) |
Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.890 | 2.080 |
High resolution limit [Å] | 2.020 | 2.020 |
Rmerge | 0.116 | 1.265 |
Rpim | 0.072 | 0.775 |
Number of reflections | 52426 | 3697 |
<I/σ(I)> | 8.3 | 1 |
Completeness [%] | 99.6 | |
Redundancy | 7 | |
CC(1/2) | 0.998 | 0.545 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 0.1-0.3M NH4 Acet. pH 5.5, 0.1-0.3M Na Acet., 25% w/v PEG3350 |