8VW4

Crystal structure of Cbl-b TKB bound to compound 26

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-03-05
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	73.376, 88.466, 118.492
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.440 - 2.400
R-factor	0.2016
Rwork	0.199
R-free	0.25310
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.694
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.440	2.486
High resolution limit [Å]	2.400	2.400
Number of reflections	30849	3014
<I/σ(I)>	11.19	1.15
Completeness [%]	99.6
Redundancy	6.5
CC(1/2)	0.996	0.488

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2 M NaCitrate, 20% PEG3350