8VVM

Structure of FabS1CE1-EPR1-1 in complex with the erythropoietin receptor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-04-06
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.979180
Spacegroup name	P 21 21 21
Unit cell lengths	73.165, 91.612, 216.735
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	84.380 - 2.900
R-factor	0.2216
Rwork	0.219
R-free	0.27270
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.217
Data reduction software	autoPROC
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	108.370	2.990
High resolution limit [Å]	2.850	2.850
Rmerge	0.122	1.043
Rmeas	0.133	1.126
Rpim	0.052	0.423
Number of reflections	34880	4546
<I/σ(I)>	10.8	1.9
Completeness [%]	99.9	100
Redundancy	6.5	7.1
CC(1/2)	0.997	0.787

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	298	0.1 M MMT buffer pH 5.0, 25% PEG1500