8VUO

Crystal structure of SARS-CoV-2 nsp16/nsp10 in complex with Cap-1 RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-08-09
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 1 21 1
Unit cell lengths	111.747, 58.893, 117.021
Unit cell angles	90.00, 95.48, 90.00

Refinement procedure

Resolution	29.700 - 2.390
R-factor	0.1819
Rwork	0.180
R-free	0.21860
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.699
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.21_5207)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.700	2.475
High resolution limit [Å]	2.390	2.390
Rmerge		1.096
Number of reflections	60283	5907
<I/σ(I)>	7.28
Completeness [%]	99.1	98.53
Redundancy	3.5	3.2
CC(1/2)	0.982	0.405

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	25% (v/v) Ethylene glycol