8VUA
Structure of FabS1CE1-EPR-1, an elbow-locked high affinity antibody for the erythropoeitin receptor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-04-06 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 72.933, 72.933, 153.366 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 58.400 - 3.270 |
| R-factor | 0.2472 |
| Rwork | 0.245 |
| R-free | 0.28230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.609 |
| Data reduction software | autoPROC |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 153.370 | 3.350 |
| High resolution limit [Å] | 3.130 | 3.130 |
| Rmerge | 0.074 | 2.017 |
| Rmeas | 0.078 | 2.121 |
| Rpim | 0.025 | 0.651 |
| Number of reflections | 8839 | 1569 |
| <I/σ(I)> | 19.5 | 1.2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 9.8 | 10.5 |
| CC(1/2) | 1.000 | 0.505 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 0.1 M HEPES 7.0, 30% Jeffamine ED-2003 |
