8VU4
Structure of FabS1CE4-EPR-1, an elbow-locked high affinity antibody for the erythropoeitin receptor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-04-06 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97918 |
Spacegroup name | P 1 |
Unit cell lengths | 55.863, 55.893, 87.016 |
Unit cell angles | 80.05, 89.91, 82.70 |
Refinement procedure
Resolution | 55.400 - 2.350 |
R-factor | 0.2105 |
Rwork | 0.208 |
R-free | 0.25160 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.063 |
Data reduction software | autoPROC |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 85.690 | 2.340 |
High resolution limit [Å] | 2.270 | 2.270 |
Rmerge | 0.115 | 0.479 |
Rmeas | 0.162 | 0.677 |
Rpim | 0.115 | 0.479 |
Number of reflections | 43559 | 4024 |
<I/σ(I)> | 8.3 | 1.7 |
Completeness [%] | 91.5 | 91.6 |
Redundancy | 2 | 1.9 |
CC(1/2) | 0.965 | 0.508 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 9 | 298 | 0.1 M BICINE 9.0, 10% PEG6000 |