8VTY

Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with ciprofloxacin and protein Y at 2.60A resolution

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-24
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97911
Spacegroup name	P 21 21 21
Unit cell lengths	209.900, 449.760, 620.450
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	122.000 - 2.600
R-factor	0.2053
Rwork	0.203
R-free	0.24130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6xhx
RMSD bond length	0.003
RMSD bond angle	0.647
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.8.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	122.000	122.000	2.670
High resolution limit [Å]	2.600	11.630	2.600
Rmerge	0.249	0.058	2.757
Rmeas	0.266	0.063	2.949
Total number of observations	13697385
Number of reflections	1772270	20606	130169
<I/σ(I)>	7.9	26.82	0.88
Completeness [%]	99.9	98.4	99.6
Redundancy	7.729	7.653	7.767
CC(1/2)	0.991	0.995	0.161

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.6	292	0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME