8VTS

Meis1 homeobox domain bound to paromomycin fragment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-12-08
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97976
Spacegroup name	P 32 1 2
Unit cell lengths	77.621, 77.621, 213.724
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.830 - 1.910
R-factor	0.1916
Rwork	0.191
R-free	0.21090
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.004
RMSD bond angle	0.489
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.930
High resolution limit [Å]	1.900	5.160	1.900
Rmerge	0.079	0.037	1.808
Rmeas	0.082	0.038	1.899
Rpim	0.023	0.010	0.573
Total number of observations	717867
Number of reflections	57573	3025	2897
<I/σ(I)>	6.8
Completeness [%]	100.0	99.9	100
Redundancy	12.5	13.4	10.7
CC(1/2)	0.997	0.999	0.532

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	2.20 M ammonium sulfate, 0.15 M sodium chloride, 20 mM Tris, 4% 2-propanol, 10 mM paromomycin, 25% ethylene glycol