8VTR
Structure of FabS1CE3-EPR-1, an elbow-locked high affinity antibody for the erythropoeitin receptor (orthorhombic form)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-04-06 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97918 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 73.199, 74.275, 93.335 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 58.120 - 1.960 |
R-factor | 0.1893 |
Rwork | 0.187 |
R-free | 0.22750 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 0.976 |
Data reduction software | autoPROC |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 74.280 | 2.010 |
High resolution limit [Å] | 1.960 | 1.960 |
Rmerge | 0.073 | 0.686 |
Rmeas | 0.079 | |
Rpim | 0.031 | 0.302 |
Number of reflections | 37275 | 2602 |
<I/σ(I)> | 18.5 | 2.3 |
Completeness [%] | 99.9 | 99.7 |
Redundancy | 6.5 | 6 |
CC(1/2) | 0.999 | 0.855 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.2 M Ammonium Citrate dibasic, 20% PEG3350. This was set up as a Fab/EPOR complex, however no EPOR was observed upon solving the structure. |