8VTN

Crystal structure of R. sphaeroides Photosynthetic Reaction Center variant Y(M210)2-nitrophenylalanine

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	250
Detector technology	PIXEL
Collection date	2023-08-16
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97893
Spacegroup name	P 31 2 1
Unit cell lengths	141.490, 141.490, 187.510
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.450 - 3.570
R-factor	0.2127
Rwork	0.211
R-free	0.24650
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.741
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.450	3.698
High resolution limit [Å]	3.570	3.570
Rmerge	0.312	1.409
Rmeas	0.328	1.543
Rpim	0.115	0.508
Number of reflections	26248	2593
<I/σ(I)>	6.66	1.46
Completeness [%]	99.5	99.92
Redundancy	7.8	8
CC(1/2)	0.994	0.659

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	1 M potassium phosphate, 3.5% 1,2,3-heptanetriol, and 0.1% LDAO precipitant solution; 1.4-1.5 M potassium phosphate reservoir solution, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K