8VS7

Crystal structure of ADI-19425 Fab in complex with anti-idiotypic 2C1 Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-07-27
Detector	DECTRIS PILATUS3 X 6M
Wavelength(s)	0.97918
Spacegroup name	P 1 21 1
Unit cell lengths	66.802, 66.930, 130.191
Unit cell angles	90.00, 103.74, 90.00

Refinement procedure

Resolution	45.960 - 2.410
R-factor	0.2025
Rwork	0.200
R-free	0.25440
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.684
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.440
High resolution limit [Å]	2.400	6.510	2.400
Rmerge	0.223	0.139	0.822
Rmeas	0.242	0.151	0.891
Rpim	0.092	0.058	0.343
Total number of observations	281056
Number of reflections	42821	2253	2099
<I/σ(I)>	6.1
Completeness [%]	99.2	99.2	99
Redundancy	6.6	6.5	6.4
CC(1/2)	0.953	0.982	0.844

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1M Imidazole pH 6.5, 12% PEG 3350, 30% MPD and 0.2M (NH4)2SO4