8VQR
Crystal structure of chimeric SARS-CoV-2 RBD complexed with chimeric raccoon dog ACE2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-1 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-10-16 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 80.961, 118.107, 112.233 |
| Unit cell angles | 90.00, 92.89, 90.00 |
Refinement procedure
| Resolution | 58.410 - 2.565 |
| R-factor | 0.2165 |
| Rwork | 0.214 |
| R-free | 0.26420 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond angle | 13.051 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 112.091 | 2.838 |
| High resolution limit [Å] | 2.565 | 2.565 |
| Rmerge | 0.049 | 0.733 |
| Rmeas | 0.057 | 0.847 |
| Rpim | 0.028 | 0.420 |
| Total number of observations | 166083 | 8531 |
| Number of reflections | 42428 | 2121 |
| <I/σ(I)> | 9.8 | 1.6 |
| Completeness [%] | 62.8 | |
| Redundancy | 3.9 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 100 mM Tris (pH 8-8.5), 18-22% PEG 6000, 100 mM NaCl and ethylene glycol (0.5-2%) |
