8VPU

Crystal structure of mouse Importin alpha in complex with dengue virus 3 NS5 central NLS peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2014-06-15
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.951
Spacegroup name	P 21 21 21
Unit cell lengths	78.920, 89.440, 100.330
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.340 - 2.100
R-factor	0.188
Rwork	0.187
R-free	0.20770
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.681
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.340	2.160
High resolution limit [Å]	2.100	2.100
Rpim	0.051	0.422
Number of reflections	40704	3382
<I/σ(I)>	11
Completeness [%]	97.6
Redundancy	4.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	296	0.75 sodium citrate, 0.1 M HEPES