8VPM

Glutaminyl cyclase ApgG, rod shape

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2022-07-18
Detector	MAR scanner 300 mm plate
Wavelength(s)	0.9787
Spacegroup name	P 64
Unit cell lengths	123.947, 123.947, 46.076
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.340 - 2.150
R-factor	0.2141
Rwork	0.212
R-free	0.24680
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.161
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	42.340	42.340	2.280
High resolution limit [Å]	2.150	6.390	2.150
Rmerge	0.086	0.056	0.730
Rmeas	0.094	0.061	0.802
Number of reflections	42880	1636	6940
<I/σ(I)>	12.39
Completeness [%]	99.6
Redundancy	11.3
CC(1/2)	0.997	0.996	0.791

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	Bis-Tris (pH 6.5), PEG 10000, and 0.2 M Potassium sodium tartrate tetrahydrate