8VON
Double phenylalanine Apex domain mutant of bacteriophage P2 central spike protein, membrane-piercing module
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-10-03 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.95370 |
Spacegroup name | P 1 |
Unit cell lengths | 45.570, 49.510, 89.540 |
Unit cell angles | 106.49, 89.74, 112.34 |
Refinement procedure
Resolution | 34.770 - 1.150 |
R-factor | 0.1145 |
Rwork | 0.114 |
R-free | 0.12760 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.098 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.180 |
High resolution limit [Å] | 1.150 | 1.150 |
Rmeas | 0.076 | 0.267 |
Number of reflections | 489332 | 34586 |
<I/σ(I)> | 13.62 | 5.55 |
Completeness [%] | 98.7 | 94 |
Redundancy | 4.81 | 4.33 |
CC(1/2) | 0.992 | 0.960 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 26-33% Pentaerythritol ethoxylate (15/4 EO/OH) aka PEE-797, 100 mM Bis-Tris or MES pH 6.0-6.5 |