8VOM

Double alanine Apex domain mutant of bacteriophage P2 central spike protein, membrane-piercing module

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2021-12-05
Detector	RAYONIX MX-300
Wavelength(s)	0.9787
Spacegroup name	P 1 21 1
Unit cell lengths	73.010, 52.810, 78.370
Unit cell angles	90.00, 108.68, 90.00

Refinement procedure

Resolution	44.060 - 1.250
R-factor	0.1194
Rwork	0.119
R-free	0.13910
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.010
Data reduction software	XDS (1.20.1_4487)
Data scaling software	XSCALE (1.20.1_4487)
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.280
High resolution limit [Å]	1.250	1.250
Rmeas	0.064	0.483
Number of reflections	306387	22084
<I/σ(I)>	18.03	5.24
Completeness [%]	99.7	97.2
Redundancy	7.42	7.16
CC(1/2)	0.997	0.959

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	26-33% Pentaerythritol ethoxylate (15/4 EO/OH) aka PEE-797, 100 mM Bis-Tris or MES pH 6.0-6.5