Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-07-18 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.570, 105.224, 186.423 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.630 - 3.000 |
| R-factor | 0.2274 |
| Rwork | 0.225 |
| R-free | 0.27000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.475 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.510 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.116 | 1.431 |
| Rmeas | 0.126 | 1.557 |
| Rpim | 0.049 | 0.608 |
| Total number of observations | 130663 | 18106 |
| Number of reflections | 19983 | 2818 |
| <I/σ(I)> | 10.1 | 1.2 |
| Completeness [%] | 99.8 | |
| Redundancy | 6.5 | 6.4 |
| CC(1/2) | 0.997 | 0.875 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 294 | 0.5M ammonium sulfate, 0.088M sodium citrate, 0.875M lithium sulfate, 2.4% glycerol, 2.5% ethylene glycol, 50mM HEPES pH 6.8 |
