8VMG

Crystal structure of GSK-3 26-383 bound to Axin 383-435

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-1
Synchrotron site	SSRL
Beamline	BL12-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-09
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97946
Spacegroup name	P 65 2 2
Unit cell lengths	195.585, 195.585, 152.204
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.760 - 2.450
R-factor	0.1953
Rwork	0.195
R-free	0.23010
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4nm0
RMSD bond length	0.006
RMSD bond angle	0.841
Data reduction software	XDS
Data scaling software	pointless (1.12.2)
Phasing software	PHENIX (1.20.1_4487)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.760	2.510
High resolution limit [Å]	2.450	2.450
Rmerge	0.222	8.110
Rmeas	0.225	8.208
Rpim	0.035	1.258
Number of reflections	63244	4331
<I/σ(I)>	15.9	0.7
Completeness [%]	99.9	98.3
Redundancy	41.1	42.2
CC(1/2)	0.999	0.414

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	298	100 mM MES, pH 5.5, 1.9 M ammonium sulfate, 200 mM sodium chloride, cryoprotectant: 25% ethylene glycol