8VMA

The crystal structure of rhinovirus C15 RNA replication element sD-loop mutant in complex with Fab BL3-6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-09-25
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.98
Spacegroup name	P 1 21 1
Unit cell lengths	52.993, 106.369, 142.819
Unit cell angles	90.00, 99.23, 90.00

Refinement procedure

Resolution	70.480 - 2.540
R-factor	0.2067
Rwork	0.205
R-free	0.24450
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.246
Data reduction software	RAPD
Data scaling software	RAPD
Phasing software	PHENIX (1.20.1_4487)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	140.970	2.620
High resolution limit [Å]	2.540	2.540
Number of reflections	51456	4415
<I/σ(I)>	12.3
Completeness [%]	99.5
Redundancy	3.5
CC(1/2)	0.999	0.425

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		298	0.2 M sodium formate, 20% w/v polyethylene glycol 3350, pH 7.2, 3.0% w/v 1,5-diaminopentane dihydrochloride linker.