8VM9

The crystal structure of rhinovirus B14 RNA replication element sD-loop mutant in complex with Fab BL3-6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-02-13
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.98
Spacegroup name	P 1 21 1
Unit cell lengths	70.921, 79.214, 141.324
Unit cell angles	90.00, 98.87, 90.00

Refinement procedure

Resolution	69.820 - 2.200
R-factor	0.2011
Rwork	0.200
R-free	0.22940
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.019
Data reduction software	RAPD
Data scaling software	RAPD
Phasing software	PHENIX (1.20.1_4487)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	139.630	2.240
High resolution limit [Å]	2.200	2.200
Rmerge	0.069
Number of reflections	77582	4411
<I/σ(I)>	11.2
Completeness [%]	98.3
Redundancy	3.5
CC(1/2)	0.997	0.662

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		298	0.1 M ammonium sulfate, 0.01 M magnesium chloride hexahydrate, 0.05 M MES monohydrate pH 5.6, 20% w/v polyethylene glycol 8000.