8VL9

Crystal structure of EloBC-VHL-CDO1 complex bound to compound 8 molecular glue

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-18
Detector	DECTRIS PILATUS 12M
Wavelength(s)	1.0001
Spacegroup name	C 2 2 21
Unit cell lengths	116.657, 177.873, 103.824
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.774 - 2.500
R-factor	0.1855
Rwork	0.184
R-free	0.21600
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.592
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.774	2.600
High resolution limit [Å]	2.500	2.500
Rmerge	0.196
Rpim	0.045
Number of reflections	37748	4247
<I/σ(I)>	10.1	0.467
Completeness [%]	100.0	100
Redundancy	19.7	15
CC(1/2)	0.997	0.567

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		294	0.2M SODIUM CITRATE, 0.1M BIS-TRIS-PROPANE PH 6.5, 20% PEG3350