8VL8
Salmonella enterica Typhimurium taxis to serine and repellents (Tsr) ligand-binding domain with L-Ser, pH 7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-05-23 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.9774 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 126.022, 74.771, 128.931 |
| Unit cell angles | 90.00, 116.14, 90.00 |
Refinement procedure
| Resolution | 61.500 - 2.500 |
| R-factor | 0.2275 |
| Rwork | 0.226 |
| R-free | 0.27340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.214 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.380 | 2.180 |
| High resolution limit [Å] | 2.120 | 2.120 |
| Number of reflections | 72723 | 4009 |
| <I/σ(I)> | 5.4 | |
| Completeness [%] | 98.3 | 88.5 |
| Redundancy | 6.1 | 4.7 |
| CC(1/2) | 0.977 | 0.568 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 1.5 ul protein at 7 mg per ml in 50 mM Tris pH 7.5, 1 mM EDTA, 150 mM NaCl, 0.5 ul of 6 mM L-serine, and 1.5 ul 1.62 M potassium phosphate pH 7 |
