8VIY

15-Lipoxygenase-2 V427L

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-22
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	H 3 2
Unit cell lengths	155.406, 155.406, 263.405
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	65.200 - 2.340
R-factor	0.186
Rwork	0.184
R-free	0.22440
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.295
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (dev_3908)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	65.200	2.430
High resolution limit [Å]	2.340	2.340
Number of reflections	51410	4909
<I/σ(I)>	16.9
Completeness [%]	99.6	96.63
Redundancy	20.3
CC(1/2)	0.999	0.602

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	0.1 M Bis-Tris pH 5.5. 1.0 (NH4)2SO4 5% glycerol 24 mM C8E4