8VGY

Crystal structure of human apoptosis-inducing factor (AIF) bound to the fused N-terminal domain of CHCHD4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-13
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.1159
Spacegroup name	P 21 21 21
Unit cell lengths	68.608, 109.197, 174.632
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	92.590 - 2.300
R-factor	0.1825
Rwork	0.181
R-free	0.21170
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5kvh
RMSD bond length	0.002
RMSD bond angle	0.544
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	92.600	2.360
High resolution limit [Å]	2.300	2.300
Rmerge	0.139	1.295
Rmeas	0.147	1.453
Rpim	0.047	0.462
Number of reflections	59115	4548
<I/σ(I)>	11.9	1.8
Completeness [%]	100.0
Redundancy	9.4
CC(1/2)	0.998	0.630

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	295	0.1 M HEPES, pH 8.0, 0.1 M NaCl,18% PEG3350