8VFS

oppF ROSE-like RNA thermometer structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-11-17
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.97741
Spacegroup name	P 43 21 2
Unit cell lengths	63.602, 63.602, 86.147
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	51.170 - 2.700
R-factor	0.213
Rwork	0.211
R-free	0.23970
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.063
Data reduction software	DIALS (3.8)
Data scaling software	DIALS (3.8)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.21rc1_5156)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	51.170	2.970
High resolution limit [Å]	2.700	2.700
Rmerge	0.096	0.712
Number of reflections	5232	1267
<I/σ(I)>	18.53	2.22
Completeness [%]	97.6
Redundancy	24
CC(1/2)	1.000	0.995

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		294	Ammonium Sulphate, Sodium acetate tri-hydrate pH 4.6, PEG MME 2000