8VFI
Ternary DNA Polymerase Beta bound to DNA containing primer terminal FapydG base-paired with a dA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-01-30 |
| Detector | DECTRIS PILATUS3 R 200K-A |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.800, 80.400, 55.500 |
| Unit cell angles | 90.00, 107.90, 90.00 |
Refinement procedure
| Resolution | 24.650 - 1.770 |
| R-factor | 0.1854 |
| Rwork | 0.181 |
| R-free | 0.22870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.291 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.800 |
| High resolution limit [Å] | 1.770 | 1.770 |
| Number of reflections | 37643 | 2006 |
| <I/σ(I)> | 14.4 | |
| Completeness [%] | 99.5 | |
| Redundancy | 3.8 | |
| CC(1/2) | 0.998 | 0.419 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 14-22% PEG 3350 0.05 M Imidazole pH 8.0 0.35 M Sodium Acetate |
