8VFC
Binary DNA Polymerase Beta bound to DNA containing primer terminal T base-paired with FapydG
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-07-07 |
Detector | DECTRIS PILATUS3 R 200K-A |
Wavelength(s) | 1.54 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 54.064, 79.728, 55.683 |
Unit cell angles | 90.00, 105.67, 90.00 |
Refinement procedure
Resolution | 24.740 - 2.480 |
R-factor | 0.2029 |
Rwork | 0.197 |
R-free | 0.25520 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.139 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.540 |
High resolution limit [Å] | 2.480 | 2.480 |
Number of reflections | 14431 | 715 |
<I/σ(I)> | 15.1 | |
Completeness [%] | 90.9 | |
Redundancy | 3.4 | |
CC(1/2) | 0.996 | 0.852 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 14-22% PEG 3350 0.05 M Imidazole pH 8.0 0.35 M Sodium Acetate |