8VF8
Binary DNA Polymerase Beta bound to DNA containing primer terminal dC base-paired with FapydG
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-05-19 |
Detector | DECTRIS PILATUS3 R 200K-A |
Wavelength(s) | 1.54 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 54.133, 79.436, 55.023 |
Unit cell angles | 90.00, 105.37, 90.00 |
Refinement procedure
Resolution | 24.790 - 1.980 |
R-factor | 0.1943 |
Rwork | 0.192 |
R-free | 0.22160 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.227 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.010 |
High resolution limit [Å] | 1.980 | 1.980 |
Number of reflections | 32838 | 1336 |
<I/σ(I)> | 12.6 | |
Completeness [%] | 95.0 | |
Redundancy | 2.6 | |
CC(1/2) | 0.989 | 0.466 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 14-22% PEG 3350 0.05 M Imidazole pH 8.0 0.35 M Sodium Acetate |