8VEW
Crystal structure of PRMT5:MEP50 in complex with MTA and oxamide compound 24
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL45XU |
| Synchrotron site | SPring-8 |
| Beamline | BL45XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-10-18 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 100.560, 137.732, 177.676 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.650 - 2.690 |
| R-factor | 0.22293 |
| Rwork | 0.220 |
| R-free | 0.26949 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.385 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.650 | 2.820 |
| High resolution limit [Å] | 2.690 | 2.690 |
| Rmerge | 0.150 | 1.326 |
| Rmeas | 0.156 | 1.380 |
| Rpim | 0.042 | 0.378 |
| Total number of observations | 59363 | |
| Number of reflections | 34631 | 4539 |
| <I/σ(I)> | 15.1 | 2.4 |
| Completeness [%] | 100.0 | |
| Redundancy | 13.5 | 13.1 |
| CC(1/2) | 0.999 | 0.902 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6 | 291 | 10% PEG 4000, 0.2M magnesium chloride, 0.1M sodium citrate (pH 6.0) |
