8VET

Crystal structure of PRMT5:MEP50 in complex with MTA and oxamide compound 1

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-02
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.07812
Spacegroup name	I 2 2 2
Unit cell lengths	103.190, 137.030, 178.880
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.700 - 2.630
R-factor	0.22691
Rwork	0.224
R-free	0.27691
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.002
RMSD bond angle	1.312
Data reduction software	XDS
Data scaling software	Aimless
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.700	2.700
High resolution limit [Å]	2.630	2.630
Rmerge	0.088	1.451
Rmeas	0.091	1.510
Rpim	0.025	0.416
Total number of observations		35949
Number of reflections	37944	2774
<I/σ(I)>	19.6	1.9
Completeness [%]	99.8
Redundancy	13.5	13
CC(1/2)	0.999	0.894

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	291	10% PEG 4000, 0.2M magnesium chloride, 0.1M MES (pH 6.5)