8VEO

Crystal structure of PRMT5:MEP50 in complex with MTA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-16
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	I 2 2 2
Unit cell lengths	101.680, 138.880, 178.370
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	54.790 - 2.030
R-factor	0.2411
Rwork	0.239
R-free	0.27750
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.011
RMSD bond angle	1.041
Data reduction software	XDS
Data scaling software	Aimless
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.790	2.080
High resolution limit [Å]	2.030	2.030
Rmerge	0.045	0.797
Rmeas	0.049	0.867
Rpim	0.019	0.338
Total number of observations	539109	36367
Number of reflections	80230	5690
<I/σ(I)>	18	2.8
Completeness [%]	98.5
Redundancy	6.7	6.4
CC(1/2)	0.999	0.914

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6	291	10% PEG 4000, 0.2M magnesium chloride, 0.1M sodium citrate (pH 6.0)