8VDV
pcsk9 in complex with inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-11-14 |
| Detector | DECTRIS EIGER R 1M |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.086, 70.846, 149.007 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 58.094 - 1.972 |
| R-factor | 0.1872 |
| Rwork | 0.186 |
| R-free | 0.21680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.520 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 149.007 | 1.978 |
| High resolution limit [Å] | 1.972 | 1.972 |
| Rmerge | 0.087 | 0.631 |
| Rmeas | 0.094 | 0.685 |
| Rpim | 0.037 | 0.264 |
| Total number of observations | 307800 | 3285 |
| Number of reflections | 47937 | 496 |
| <I/σ(I)> | 15.1 | 2.8 |
| Completeness [%] | 100.0 | |
| Redundancy | 6.4 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 298 | 20.0% Peg-6000, 0.1M Tris pH8.0 |
